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24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu Logo Principal UB AgroSup CNRS



Professeur of biochemistry (PR-EX, section 64)
Pôle : Plant Microorganism Interaction
Equipe/Thème : Nitric Oxyde, signaling and plant immunity
Courriel :
Tel :

Curriculum Vitae


Since 2012: Group leader of the research team “Nitric Oxide and Plant Immunity", member of the editorial boards of Journal of Biological chemistry and Plant Science. 

2007-2011 : Professor of Biochemistry. Group leader of the research team “Signalling in plant defence responses" in the Plant-Microbe-Environment Research Unit.

2004 : Habilitation to conduct Researches (HDR).

1998-2007 : Associate Professor in Biochemistry, University of Burgundy, France. Research activities in the Plant-Microbe-Environment Research Unit (headed by Dr. V. Gianinazzi-Pearson).

1996-98 : Post-doctoral fellowship, Waksman Institute, University of Rutgers, NJ, USA (group leader : Pr. D. F. Klessig).

1992-96 : PhD degree in Biochemistry, University of Burgundy, France (group leader Pr. A. Pugin)

Research Activities

A major objective of our lab is to understand how plants respond to pathogenic attack at the molecular and cellular levels. During the past years, we have attempted to identify and characterize signalling proteins mobilized in plant cells exposed to pathogenic fungi, effectors and MAMPs (Microbe-Associated Molecular Patterns). Proteins of interest include type II histones déacetylases, SNF1-related protein kinases type 2 and proteins regulated at the posttranslational level by NO through S-nitrosylation including for instance calmodulin and the chaperon-like protein Cdc48. The regulation, as well as the role of these proteins in plant facing pathogen attacks has been called into questions. In a parallel project, we also investigate the interplays operating between nitrogen and iron nutrition/homeostasis and plant immunity. Finally, we are also interested in developing applied researches aimed at stimulating plant natural defences.

Activités d'enseignement

I’m teaching Biochemistry (192 h /year) in the following teaching units :
- Molecules of living organisms : structures, functions, analysis and applications (first year of bachelor of Technics and Sciences)
- Structure and function of proteins and metabolic regulation (third year of Bachelor of Biology)
- Bioactive molecules (first year of Master Life Sciences)
- Cellular dynamic and signal transduction (first year of Master Life Sciences)
- Scientific culture (first year of Master Life Sciences)
- Cellular and Molecular Signaling (Master 2 of Research “Cellular and Molecular Signaling”)
- Director of the master 2 of Research “Cellular and Molecular Signaling”



  • Kulik A., Noirot E., Grandperret V., Bourque S., Fromentin J., Salloignon P., Truntzer C., Dobrowolska G., Simon-Plas F. and Wendehenne D. 2015. Interplays between nitric oxide and reactive oxygen species in cryptogein signaling. Plant Cell Environ. 38, 331–348.
  • Trapet P., Kulik A., Lamotte O., Jeandroz S., Bourque S., Nicolas-Francès V., Rosnoblet C., Besson-Bard A. and Wendehenne D. 2015. NO signaling in plant immunity: a tale of messengers. Phytochem. 112, 72-79 (special issue in honour of Paul Bolwell).
  • Lamotte O., Bertoldo J., Besson-Bard A., Rosnoblet C., Aimé S., Hichami S., Terenzi H. and Wendehenne D. 2015. Protein S-nitrosylation: specificity and identification strategies in plants. Front. Chem. 2:114. 


  • Koen E., Trapet P., Brulé D., Kulik A., Klinguer A., Atauri-Miranda L., Meunier-Prest R., Boni G., Glauser G., Mauch-Mani B., Wendehenne D. and Besson-Bard A. 2014. β-aminobutyric acid (BABA)-induced resistance in Arabidopsis thaliana: link with iron homeostasis. Mol Plant-Microbe Interact. 27, 1226-1240.
  • Wendehenne D., Gao Q-M., Kachroo A. and Kachroo P. 2014. Free radical-mediated systemic immunity in plants. Curr. Opin. Plant Biol. 20, 127–134.
  • Wang C., El-Shetehy M., Shine M.B., Yu K., Navarre D., Wendehenne D., Kachroo A. and Kachroo P. 2014. Free radicals mediate systemic acquired resistance. Cell Rep. 7, 348-355.
  • Grandperret V., Nicolas-Francès V., Wendehenne D. and Bourque S. 2014. Type-II Histone DeACetylases: elusive plant nuclear signal transducers. Plant Cell Environ. 37, 1259-1269.
  • Gauthier A., Trouvelot S., Kelloniemi J., Frettinger P., Dubreuil-Maurizi C., Wendehenne D.,  Daire X., Joubert J-M., Ferrarini A., Delledonne M., Flors V. and Poinssot B. 2014. The sulfated laminarin triggers a stress transcriptome before priming the SA- and ROS-dependent defenses during grapevine's induced resistance against Plasmopara viticola. PLoS ONE 9, e88145.


  • Jeandroz S., Lamotte O., Astier J., Rasul S., Trapet P., Besson-Bard A., Bourque S., Nicolas-Francès V., Ma W., Berkowitz G.A. and Wendehenne D. 2013. There's more to the picture than meets the eye: Nitric oxide cross-talk with Ca2+ signaling. Plant Physiol. 163, 459-470.
  • Manzoor H., Kelloniemi J., Chiltz A., Wendehenne D., Pugin A., Poinssot B. and Garcia-Brugger A. 2013. Involvement of the glutamate receptor AtGLR3.3 in plant defense signaling and resistance to Hyaloperonospora arabidopsis. Plant J. 76, 466–480.
  • Koen E., Besson-Bard A., Duc C., Astier J., Gravot A., Richaud P., Lamotte O., Boucherez J., Gaymard F. and Wendehenne D. 2013. Arabidopsis thaliana nicotianamine synthase 4 is required for proper response to iron deficiency and to cadmium exposure. Plant Sci. 209, 1-11.
  • Koen E., Lamotte O., Besson-Bard A., Bourque S., Nicolas-Francès V., Jeandroz S. and Wendehenne D. 2013. Le monoxyde d'azote, un acteur de l'immunité chez les plantes. Med. Sci. 29, 306-316.



  • Dubreuil-Maurizi C., Vitecek J., Marty L., Branciard L., Frettinger P., Wendehenne D., Meyer A.J., Mauch F. and Poinssot B. 2011. Glutathione deficiency of the Arabidopsis mutant pad2-1 affects oxidative stress-related events, defense gene expression and hypersensitive response. Plant Physiol. 157, 2000-2012. (FI: 6,53).
  • La Camera S., L'Haridon F., Astier J., Zander M., Abou-Mansour E., Page G., Thurow C., Wendehenne D., Gatz C, Métraux J-P. and Lamotte O. 2011. The glutaredoxin ATGRXS13 is required for Arabidopsis susceptibility to the necrotrophic fungus Botrytis cinerea. Plant J. 68, 507-519 (FI: 6,16).
  • Bourque S., Dutartre A., Hammoudi V., Blanc S., Dahan J., Jeandroz J., Pichereaux C., Rossignol M. and Wendehenne D. 2011. Type-2 histone deacetylases as new regulators of elicitor-induced cell death in plants. New Phytol. 192, 127-139 (FI: 6,64).
  • Vatsa P, Chiltz A., Bourque S., Wendehenne D., Garcia-Brugger A. and Pugin A. 2011. Involvement of putative glutamate receptors in plant defence signaling and NO production. Biochimie 93, 2095-2101 (FI: 3.02).
  • Astier J., Rasul S., Koen E., Manzoor H., Besson-Bard A., Lamotte O., Jeandroz S., Durner J., Lindermayr C. and Wendehenne D. 2011. S-nitrosylation: an emerging post-translational protein modification in plants. Plant Science 181, 527-533 (FI: 2,94).
  • Gamm M., Héloir M.C., Kelloniemi J., Poinssot B., Wendehenne D. and Adrian M. 2011. Identification of reference genes suitable for qRT-PCR in grapevine and application for the study of the expression of genes involved in pterostilbene synthesis. Molecular Genetics and Genomics 285, 273-285. (FI: 2,63).
  • Gamm M., Héloir M-C., Bligny R., Vaillant-Gaveau N., Trouvelot S., Alcaraz G., Frettinger P., Clément C., Pugin A., Wendehenne D. and Adrian M. 2011. Changes in carbohydrate metabolism in Plasmopara viticola infected grapevine leaves. Mol. Plant-Microbe-Interact. 24, 1061-1073. (FI: 4,43).


  • Selim S., Negrel J., Wendehenne D., Ochatt S., Gianinazzi S. and van Tuinen D. 2010. Stimulation of defence reactions in Medicago truncatula by antagonistic lipopeptides from Paenibacillus sp. strain B2. Appl. Environ. Microbiol. 76, 7420-7428. (FI: 3,78).
  • Wawer I., Bucholc M., Astier J., Anielska-Mazur A., Dahan J., Kulik A., Wysłouch-Cieszynska A., Zareba-Kozioł M., Krzywinska E., Dadlez M., Dobrowolska G. and Wendehenne D. 2010. Regulation of Nicotiana tabacum osmotic stress-activated protein kinase and its cellular partner GAPDH by nitric oxide in response to salinity. Biochem J. 429, 73-83. (FI: 5).
  • Dahan J., Wendehenne D., Ranjeva R., Pugin A. and Bourque S. 2010. Nuclear protein kinases: still enigmatic components in plant cell signalling. New Phytol. 185, 355-368. (FI: 6,51).
  • Dubreuil C., Trouvelot S., Frettinger P., Pugin A., Wendehenne D. and Poinssot B. 2010. β-Aminobutyric Acid Primes a NADPH Oxidase-Dependent Reactive Oxygen Species Production during Grapevine Triggered Immunity. Mol. Plant-Microbe-Interact. 23, 1012-21. (FI: 4).


  • Dahan J., Pichereaux C., Rossignol M., Blanc S., Wendehenne D., Pugin A. and Bourque S. 2009. Activation of a nuclear localized-SIPK in tobacco cells challenged by cryptogein, an elicitor of plant defence reactions. Biochem J. 418, 191-200. (FI: 5,15).
  • Besson-Bard A., Gravot A., Richaud P., Auroy P., Duc C., Gaymard F., Taconnat L., Renou J-P., Pugin A. and Wendehenne D. 2009. Nitric oxide contributes to cadmium toxicity in Arabidopsis thaliana by promoting cadmium accumulation in roots and by up-regulating genes related to iron uptake. Plant Physiol. 149, 1302-1315. (FI: 6,2).
  • Allègre M., Héloir M.-C., Trouvelot S., Daire X., Pugin A., Wendehenne D. and Adrian M. 2009. Are grapevine stomata involved in the elicitor-induced protection against downy mildew? Mol. Plant Microbe Interact. 22, 977-986. (FI: 4,4).
  • Besson-Bard A., Astier J., Rasul S., Wawer I., Dubreuil-Maurizi C., Jeandroz S. and Wendehenne D. 2009. Current view of nitric oxide-responsive genes in plants. Plant Sci. 177, 302–309. (FI: 2,05; article sur invitation en tant que nouveau membre de l’Editorial Board de Plant Science).


  • Courtois C., Besson-Bard A., Dahan J., Bourque S., Dobrowolska G., Pugin A. and Wendehenne D. 2008. Nitric oxide signalling in plants: interplays with Ca2+ and protein kinase. J. Exp. Bot. 59, 155-163. (FI : 4).
  • Besson-Bard A., Courtois C., Gauthier A., Dahan J., Dobrowolska G., Jeandroz S., Pugin A. and Wendehenne D. (2008). Nitric oxide in plants: production and cross-talk with Ca2+ signalling. Mol. Plant 1, 218-228. (4,3).
  • Besson-Bard A., Pugin A and Wendehenne D. 2008. New Insights into nitric oxide signaling in plants. Annu. Rev. Plant Biol. 59, 21-39. (FI:  22,2).
  • Besson-Bard A., Griveau S., Bedioui F. and Wendehenne D. 2008. Real-time electrochemical detection of extracellular nitric oxide in tobacco cells exposed to cryptogein, an elicitor of defence responses. J. Exp. Bot. 59, 3407–3414. (FI : 4).
  • van Loon L.C., Bakker P.A.H.M., van der Heijdt W.H.W., Wendehenne D. and Pugin A. 2008. Early responses of tobacco suspension cells to rhizobacterial elicitors of induced systemic resistance. Mol. Plant Microbe Interact. 21, 1609-21. (FI: 4,2).
  • Errakhi R., Dauphin A., Meimoun P., Lehner A., Reboutier D., Vatsa P., Briand J., Madiona K., Rona J.-P., Barakate M., Wendehenne D., Beaulieu C. and Bouteau F. 2008. Thaxtomin A-induced defense responses in Arabidopsis thaliana cells require an early Ca2+ influx. J. Exp. Bot. 59, 4259-4270. (FI: 4).


  • Gauthier A., Lamotte O., Reboutier D., Bouteau F., Pugin A. and Wendehenne D. 2007. Cryptogein-induced anion effluxes: electrophysiological properties and analysis of the mechanisms through which they contribute to the elicitor-triggered cell death. Plant Signal. Behav. 2, 89-98. 


  • Vandelle E., Poinssot B., Wendehenne D., Bentejac M. and Pugin A. 2006. Integrated signaling network involving calcium, nitric oxide, active oxygen species but not mitogen-activated protein kinases in BcPG1-elicited grapevine defenses. Mol. Plant Microbe Interact. 19, 429-440. (FI: 3,9).
  • Lamotte O., Courtois C., Dobrowolska G., Besson A., Pugin A. and Wendehenne D. 2006. Mechanisms of nitric oxide-induced increase of free cytosolic Ca2+ concentration in Nicotiana plumbaginifolia cells. Free Rad. Biol. Med. 40, 1369-1376. (FI: 5,4). 
  • Garcia-Brugger A., Lamotte O., Vandelle E., Bourque S., Lecourieux D., Poinssot B., Wendehenne D. and Pugin A. 2006. Signaling pathways activated by elicitors of plant defenses. Mol. Plant Microbe Interact. 19, 711-724. (FI: 3,9).
  • Une des images de cet article illustre la couverture de la revue.
  • Conrath U., Beckers G.J.M, Flors V., Garcia-Agustin P., Jakab G., Mauch F., Mauch-Mani B., Newman M.-A., Pieterse C.M.J., Poinssot B., Pozo M.J., Pugin A., Schaffrath U., Ton J., Wendehenne D., Zimmerli L. 2006. Priming: getting ready for battle. Mol. Plant Microbe Interact. 19, 1062-1071. (FI: 3,9).


  • Lecourieux D., Lamotte O., Bourque S., Mazars C., Wendehenne D., Ranjeva R. and Pugin A. 2005. Elicitors induce specific changes in nuclear free calcium in tobacco cell suspensions. Cell Calcium 38, 527-538. (FI: 5,2).
  • Lamotte O., Courtois, C., Barnavon L., Pugin, A. and Wendehenne D. 2005. Nitric oxide in plants : the biosynthesis and cell signalling properties of a fascinating molecule. Planta 221, 1-4. (FI: 3,1).


  • Lamotte O., Gould K., Lecourieux D., Sequeira-Legrand A., Lebrun-Garcia A., Durner J., Pugin A. and Wendehenne D. 2004. Analysis of nitric oxide signalling functions in tobacco cells challenged by the elicitor cryptogein. Plant Physiol. 135, 516-529. (FI: 5,9).
  • Wendehenne D., Durner J. and Klessig D. 2004. Nitric oxide : a new player in plant signaling and defence responses. Curr. Opin. Plant Biol. 7, 449-455. (FI: 9).


  • Gould K., Lamotte O., Klinguer A., Pugin A. and Wendehenne D. 2003. Nitric oxide production by tobacco leaves : a general stress responses ? Plant Cell Environ. 26, 1851-1862. (FI: 3,6). Une des images de microscopie obtenues dans ce travail illustre la couverture de la revue.


  • Wendehenne D., Lamotte O., Frachisse J.-M., Barbier-Brygoo H. and Pugin A. 2002. Nitrate efflux is an essential component of the cryptogein signaling pathway leading to defense responses and hypersensitive cell death in tobacco. Plant Cell 14, 1937-1951. (FI: 11,8).Un commentaires relatifs à cet article a été publié dans "Biology Reports Ltd Faculty of 1000". 


  • Wendehenne D., Pugin A., Klessig D. and Durner J. 2001. Nitric oxide: comparative synthesis and signaling in animal and plant cells. Trends Plant Sci. 6, 177-183. (FI: 11,4).


  • Navarre D.A., Wendehenne D., Durner J., Noad R. and Klessig D.F. 2000. Nitric oxide modulates the activity of tobacco aconitase. Plant Physiol. 122, 573-582. (FI: 4,8).
  • Klessig D.F., Durner J., Noad R., Navarre D.A., Wendehenne D., Kumar D., Zhou J.M., Shah J., Zhang S., Kachroo P., Trifa Y., Pontier D., Lam E. and Silva H. 2000. Nitric oxide and salicylic acid signaling in plant defense. Proc. Natl. Acad. Sci. USA 97 8849-8855. (FI: 10,8).
  • Foissner I., Wendehenne D., Langebartels  C. and Durner J. 2000. In vivo imaging of an elicitor-induced nitric oxide burst in tobacco. Plant J. 23,  817-824. (FI: 5,6). Une des images de microscopie obtenues dans ce travail illustre la couverture de la revue.


  • Lebrun-Garcia A., Bourque S., Binet M.-N., Ouaked F., Wendehenne D., Chiltz A., Shaffner A. and Pugin A. 1999. Involvement of plasma membrane proteins in plant defense responses. Biochimie 81, 1-6. (FI: 1,6). * 


  • Durner J., Wendehenne D. and Klessig D.F. 1998. Defense gene induction in tobacco by nitric oxide, cyclic GMP, and cyclic ADP-ribose. Proc. Natl. Acad. Sci. USA 95, 10328-10333. (FI: 9,8).
  • Des commentaires relatifs à cet article ont été publiés par J. Dangl (Nature 394, 525-527, 1998) et Hausladen and Stamler (Proc. Natl. Acad. Sci. USA 95, 10345-10347, 1998).
  • Wendehenne D., Durner J., Chen Z. and Klessig D.F. 1998. Benzothiadiazole, an inducer of plant defenses, inhibits catalase and ascorbate peroxidase. Phytochemistry 47, 651-657. (FI: 1,2).


  • Wendehenne D., Binet M.-N., Blein J.-P., Ricci P. and Pugin A. 1995. Evidence for specific, high-affinity binding sites for a proteinaceous elicitor in tobacco plasma membrane. FEBS Lett. 374, 203-207. (FI: 3,8).
  • Tavernier E., Wendehenne D., Blein J.-P. and Pugin A. 1995. Involvement of free calcium in action of cryptogein, a proteinaceous elicitor of hypersensitive reaction in tobacco cells. Plant Physiol. 109, 1025-1031. (FI: 3,8).


Key words

Plant immunity, Signalling, Posttranslational protein Modifications, nitric oxide, iron homeostasis